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Sangon Biotech sanprep column plasmid mini preps kit
Sanprep Column Plasmid Mini Preps Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanprep+column+plasmid+mini+preps+kit/column+dna+extraction+gel+kit+sanprep/pmc12907642-86-9-25
Average 86 stars, based on 1 article reviews
sanprep column plasmid mini preps kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Plasmid Preparation:

Article Title: A New Strategy to Identify Naturally Presenting SLA-I Bound Peptides Derived from the O Serotype of Foot-and-Mouth Disease Virus, by Mild Acid Elution in a VP1 Stably Expressed PK15 Cell Line
Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco Company (Boston, MA, USA). .. A horseradish peroxidase conjugated goat anti-mouse Immunoglobulin G (IgG) monoclonal antibody, the SanPrep Column Plasmid Mini-Preps Kit, and the SanPrep Column DNA Gel Extraction Kit were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). .. The reverse transcription kit, T4 DNA Ligase, and Taq polymerase were purchased from TaKaRa Biotechnology Co., Ltd. (Dalian, China).

Article Title: TaqMan qPCR quantification of the Cytb gene for precise determination of camel milk in dairy products
Article Snippet: .. The recombinant plasmids were then extracted using the SanPrep Column Plasmid Mini-Preps Kit (Sangon, Cat. #B518191) and samples were sent to Sangon Biotech Co., Ltd (Shanghai, China) for Sanger sequencing. ..

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system.
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis
Article Snippet: SanPrep Column DNA Gel Extraction Kit , Sangon Biotech , Cat#B518131. .. SanPrep Column Plasmid Mini-Preps Kit , Sangon Biotech , Cat#B518191. .. ECL Enhanced Kit , ABclonal , Cat#RM00021.

Article Title: Multi-enzyme cascade colouring for meat without nitrite at low-temperature: Catalysis by NOS-YkuN-YumC and glucose dehydrogenase.
Article Snippet: This study investigated the role of a multi-enzyme cascade system in developing the colour of meat products at 10 C. The system consists of glucose dehydrogenase (GDH) and a fusion enzyme, NOS-YkuN-YumC, which was created through the genetic fusion of the NOS gene to those encoding the flavonoid-binding protein YkuN and the flavonoid reductase YumC.. The results showed that recombinant GDH was successfully expressed and purified from Bacillus subtilis 168.. The purified GDH exhibited a molecular weight of approximately 32 kDa and a specific activity of 11.40 U/mg, demonstrating high efficiency in nicotinamide adenine dinucleotide phosphate (NADPH) regeneration.

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

Article Title: The Effects of Feeding ybfQ -Deficient Gut Bacteria on Radio-Tolerance in Symbiotic Caenorhabditis elegans : The Key Role of Isoscoparin
Article Snippet: .. Plasmids were extracted from these colonies using the SanPrep Column Plasmid Mini-Preps Kit (Cat. No. B518191, Sangon Biotech) and introduced into competent cells of both BW and ΔybfQ bacterial strains to generate overexpression lines. ..

Article Title: Engineering L-threonine transaldolase and a chaperone assisted cascade for the efficient biosynthesis of L-threo-p-methylsulfonylphenylserine
Article Snippet: The efficient asymmetric synthesis of L-threo-p-methylsulfonylphenylserine (L-threo-4-MTPS) is frequently hindered by the limited catalytic efficiency of natural transaldolases and severe feedback inhibition caused by acetaldehyde accumulation.. In this study, we identified a novel L-threonine transaldolase from Chitiniphilus eburneus (CeLTTA) via systematic genome mining.. To overcome its kinetic limitations, structure-guided semi-rational evolution was employed, yielding the triple mutant F70N/F59A/C57M with 4.46-fold increase in specific activity and maintained superior stereocontrol.

Gel Extraction:

Article Title: A New Strategy to Identify Naturally Presenting SLA-I Bound Peptides Derived from the O Serotype of Foot-and-Mouth Disease Virus, by Mild Acid Elution in a VP1 Stably Expressed PK15 Cell Line
Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco Company (Boston, MA, USA). .. A horseradish peroxidase conjugated goat anti-mouse Immunoglobulin G (IgG) monoclonal antibody, the SanPrep Column Plasmid Mini-Preps Kit, and the SanPrep Column DNA Gel Extraction Kit were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). .. The reverse transcription kit, T4 DNA Ligase, and Taq polymerase were purchased from TaKaRa Biotechnology Co., Ltd. (Dalian, China).

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system.
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

Recombinant:

Article Title: TaqMan qPCR quantification of the Cytb gene for precise determination of camel milk in dairy products
Article Snippet: .. The recombinant plasmids were then extracted using the SanPrep Column Plasmid Mini-Preps Kit (Sangon, Cat. #B518191) and samples were sent to Sangon Biotech Co., Ltd (Shanghai, China) for Sanger sequencing. ..

Article Title: Multi-enzyme cascade colouring for meat without nitrite at low-temperature: Catalysis by NOS-YkuN-YumC and glucose dehydrogenase.
Article Snippet: This study investigated the role of a multi-enzyme cascade system in developing the colour of meat products at 10 C. The system consists of glucose dehydrogenase (GDH) and a fusion enzyme, NOS-YkuN-YumC, which was created through the genetic fusion of the NOS gene to those encoding the flavonoid-binding protein YkuN and the flavonoid reductase YumC.. The results showed that recombinant GDH was successfully expressed and purified from Bacillus subtilis 168.. The purified GDH exhibited a molecular weight of approximately 32 kDa and a specific activity of 11.40 U/mg, demonstrating high efficiency in nicotinamide adenine dinucleotide phosphate (NADPH) regeneration.

Sequencing:

Article Title: TaqMan qPCR quantification of the Cytb gene for precise determination of camel milk in dairy products
Article Snippet: .. The recombinant plasmids were then extracted using the SanPrep Column Plasmid Mini-Preps Kit (Sangon, Cat. #B518191) and samples were sent to Sangon Biotech Co., Ltd (Shanghai, China) for Sanger sequencing. ..

Extraction:

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system.
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

Article Title: Multi-enzyme cascade colouring for meat without nitrite at low-temperature: Catalysis by NOS-YkuN-YumC and glucose dehydrogenase.
Article Snippet: This study investigated the role of a multi-enzyme cascade system in developing the colour of meat products at 10 C. The system consists of glucose dehydrogenase (GDH) and a fusion enzyme, NOS-YkuN-YumC, which was created through the genetic fusion of the NOS gene to those encoding the flavonoid-binding protein YkuN and the flavonoid reductase YumC.. The results showed that recombinant GDH was successfully expressed and purified from Bacillus subtilis 168.. The purified GDH exhibited a molecular weight of approximately 32 kDa and a specific activity of 11.40 U/mg, demonstrating high efficiency in nicotinamide adenine dinucleotide phosphate (NADPH) regeneration.

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

Over Expression:

Article Title: The Effects of Feeding ybfQ -Deficient Gut Bacteria on Radio-Tolerance in Symbiotic Caenorhabditis elegans : The Key Role of Isoscoparin
Article Snippet: .. Plasmids were extracted from these colonies using the SanPrep Column Plasmid Mini-Preps Kit (Cat. No. B518191, Sangon Biotech) and introduced into competent cells of both BW and ΔybfQ bacterial strains to generate overexpression lines. ..

Polymerase Chain Reaction:

Article Title: Engineering L-threonine transaldolase and a chaperone assisted cascade for the efficient biosynthesis of L-threo-p-methylsulfonylphenylserine
Article Snippet: The efficient asymmetric synthesis of L-threo-p-methylsulfonylphenylserine (L-threo-4-MTPS) is frequently hindered by the limited catalytic efficiency of natural transaldolases and severe feedback inhibition caused by acetaldehyde accumulation.. In this study, we identified a novel L-threonine transaldolase from Chitiniphilus eburneus (CeLTTA) via systematic genome mining.. To overcome its kinetic limitations, structure-guided semi-rational evolution was employed, yielding the triple mutant F70N/F59A/C57M with 4.46-fold increase in specific activity and maintained superior stereocontrol.

Purification:

Article Title: Engineering L-threonine transaldolase and a chaperone assisted cascade for the efficient biosynthesis of L-threo-p-methylsulfonylphenylserine
Article Snippet: The efficient asymmetric synthesis of L-threo-p-methylsulfonylphenylserine (L-threo-4-MTPS) is frequently hindered by the limited catalytic efficiency of natural transaldolases and severe feedback inhibition caused by acetaldehyde accumulation.. In this study, we identified a novel L-threonine transaldolase from Chitiniphilus eburneus (CeLTTA) via systematic genome mining.. To overcome its kinetic limitations, structure-guided semi-rational evolution was employed, yielding the triple mutant F70N/F59A/C57M with 4.46-fold increase in specific activity and maintained superior stereocontrol.



Similar Products

86
Sangon Biotech sanprep column plasmid mini preps kit
Sanprep Column Plasmid Mini Preps Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanprep+column+plasmid+mini+preps+kit/column+dna+extraction+gel+kit+sanprep/pmc12907642-86-9-25
Average 86 stars, based on 1 article reviews
sanprep column plasmid mini preps kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Sangon Biotech sanprep column plasmid mini prep kit
Sanprep Column Plasmid Mini Prep Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanprep+column+plasmid+mini+preps+kit/column+dna+extraction+gel+kit+sanprep/pm42042332-95-7-26
Average 86 stars, based on 1 article reviews
sanprep column plasmid mini prep kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

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